Photometry

➔ Advice prior to purchasing equipment and supplies suited to your experiments.

➔ Installation and testing of your photometry setup.

➔ Synchronization of your photometry equipment with your various behavioural apparatus.

➔ The GCaMP protein (excitation and emission), how to correct for photobleaching.

➔ Understand the different recordings systems and accessories.

➔ Principles of analysis: understanding the isosbestic signal, correcting artifacts, and calculating dF/F.

➔ Introduction to brain surgery and implant placement.

➔ Calcium signal recordings using the principal photometry systems (Doric, RWD, Thorlabs/Hamamatsu).

➔ Personalized support for your first photometry study.

➔ Assistance with recordings, discussion of your behavioural task, advice on the overall approach.

➔ Joint analysis of the obtained results.